Quick Neuron™ Cholinergic – Human iPSC-Derived Neurons (Healthy Donor)
Quick-Neuron™ human iPSC-derived cholinergic neurons are off-the-shelf neurons that provide a consistent, biologically relevant in vitro model of human cholinergic neuronal function. These cryopreserved, ready-to-use iPSC-derived neurons mature rapidly in culture and deliver reproducible performance for studying cholinergic signaling, synaptic activity, neuronal network development, neurodegenerative disease research, drug discovery, and neurotoxicity screening.
$850.00
Advantages of iPSC-Derived Cholinergic Neurons
Cholinergic Neuron Protocol
Explore our detailed differentiation protocols, a step-by-step guide designed to simplify and optimize your laboratory procedures using our iPSC-derived cells and differentiation kits. These protocols leverage the latest advancements in iPSC technology to ensure efficient and reproducible results.
Cholinergic Neuron Characterization
Characterization of iPSC-derived cholinergic neurons is crucial to ensure their utility in research. Employing cholinergic neuron markers, we can confirm the identity and purity of these neurons.
Marker Expression
Understanding the role of cholinergic neuron markers is crucial in neuroscience research. Our comprehensive guide delves into the identification and significance of these markers in iPSC-derived neurons, providing essential information for researchers.
Immunocytochemistry (ICC)
iPSC-derived cholinergic neurons express neuronal markers and display typical neurite growth. Immunofluorescent staining of Quick-Neuron™ Cholinergic – Human iPSC-derived Neurons on Day 3 post-thaw that shows expression of the pan-neuronal marker TUBB3 and the cholinergic neuron marker ChAT.
Lot-to-Lot Consistency
Consistency across production lots supports reproducible results in long-term studies and screening campaigns. Three independent production lots of Quick-Neuron™ Cholinergic Neurons were evaluated for post-thaw viability, Day 1 viability, and neuronal marker expression to assess consistency from lot to lot.
- Lot 5911
- Lot 5912
- Lot 5934
- Range
- Mean
| Parameter | Individual lot measurements, percentage scale | Mean | Range | CV |
|---|---|---|---|---|
| Post-thaw viability | 89.4% | 87.0–93.0% | 3.5% | |
| D1 viability | 83.3% | 78–86% | 5.5% | |
| TUBB3+ | 98.1% | 97.3–98.6% | 0.7% | |
| ChAT+ / TUBB3+ | 58.0% | 53.9–64.0% | 9.1% |
Lot-to-lot consistency of Quick-Neuron™ Cholinergic Neurons. Post-thaw viability, Day 1 viability, percentage of TUBB3+ neurons, and percentage of ChAT+ cells among TUBB3+ neurons across three independent production lots (5911, 5912, and 5934). Each point represents one lot; the shaded band spans the range across lots and the red line marks the mean. CV = coefficient of variation.
Additional Cholinergic Neuron Data
ALS Disease Study
Mislocalization of TDP-43 from the nucleus to the cytoplasm is a pathological feature observed in most ALS cases, making it a widely studied readout in ALS disease modeling. Here, iPSCs from a healthy control donor and an ALS patient donor were differentiated into cholinergic neurons using the Quick-Neuron™ Cholinergic – SeV Kit and examined for TDP-43 localization.
TDP-43 localization in healthy control and ALS patient-derived cholinergic neurons. iPSCs from a healthy control donor and an ALS patient donor were differentiated into cholinergic neurons and fixed at Day 10 post-differentiation. Cultures were co-stained for the pan-neuronal marker TUBB3 (green) and TDP-43 (red). Representative images are shown. Punctate TDP-43 signal outside the nucleus was observed in ALS patient-derived neurons.
Neurite Length Analysis
Neurite length is a quantitative, image-based readout commonly used to assess neuronal morphology and outgrowth. Because it can be measured from label-free live-cell images, the same cultures can be monitored over time. Neurite length measurements may be useful for comparing culture conditions, such as healthy versus patient-derived lines or treated versus untreated cultures.
Label-free monitoring of neurite outgrowth over time. (Left) Digital phase contrast (DPC) image of Quick-Neuron™ Cholinergic cells at Day 5, with image-analysis overlays marking somata (blue) and neurites (green); scale bar, 100 µm. (Right) Neurite length measured over 58 days for samples E_02–E_11.
Product Specifications
| Parameters | Specifications |
|---|---|
| Product Name | Quick-Neuron™ Cholinergic - Human iPSC-Derived Neurons |
| Catalog No. | CH-SeV-HC-CW50065 |
| Product Components | Cryopreserved cells, Component N1, Component A, and Component P |
| Starting Material | iPSCs derived from peripheral blood mononuclear cells (CIRM line CW50065) |
| Storage Conditions | Frozen cells should be stored in liquid nitrogen (vapor phase). The rest of the components should be stored at -20°C. |
| Cell Type | Cholinergic Neurons |
| Culture Type | Feeder Cell-Free |
| Disease | Healthy Donor |
| Donor Sex | Female |
| Donor Age at Sampling | 74 |
| Donor Race Ethnicity | Caucasian, not Latino |
| Patient History | See Resources for more information. |
| Reprogramming Method | Episomal vector |
| Induction Method | Transcription factors delivered by Sendai virus |
| Growth Properties | Adherent |
| Number of viable cells | > 1.0 million viable cells per vial upon thawing |
| Cell viability and remaining live cells | >70% at day 1, >211 live cells per mm2 >50% at day 7, >211 live cells per mm2 |
| Differentiation | >80% TUBB3 positive cells >50% ChAT positive cells among TUBB3 positive cells |
| Sterility | No growth observed |
| Mycoplasma | No mycoplasmal enzymes detected |
| Morphological Observation | Cells are adherent and neurites exhibit substantial outgrowth, elongation and branching, indicative of a differentiated phenotype. |
| Restricted Use | For research use only. Not for use in diagnostic or therapeutic procedures. |
