Quick Neuron™ Cholinergic – SeV Kit
Quick-Neuron™ Cholinergic – SeV Kits enable the differentiation of human pluripotent stem cells into cholinergic neurons in as little as 7 days using Sendai virus technology.
Note: This kit is no longer available for individual sale. We now offer bulk orders and custom differentiation services to support your research needs.
Advantages of iPSC-Derived Cholinergic Neurons
Cholinergic Neuron Differentiation Kit Protocol
Explore our detailed differentiation protocols, a step-by-step guide designed to simplify and optimize your laboratory procedures using our iPSC-derived cells and differentiation kits. These protocols leverage the latest advancements in iPSC technology to ensure efficient and reproducible results.
Representative workflow for Quick-Neuron™ Cholinergic – SeV Kit
Cholinergic Neuron Characterization
Characterization of iPSC-derived cholinergic neurons is crucial to ensure their utility in research. Employing cholinergic neuron markers, we can confirm the identity and purity of these neurons.
Cholinergic Neuron Marker Expression
Understanding the role of cholinergic neuron markers is crucial in neuroscience research. Our comprehensive guide delves into the identification and significance of these markers in iPSC-derived neurons, providing essential information for researchers.
iPSC-derived cholinergic neurons express neuronal markers and display typical neurite growth. Immunofluorescent staining of Quick-Neuron™ Cholinergic – Human iPSC-derived Neurons on Day 3 post-thaw that shows expression of the pan-neuronal marker TUBB3 and the cholinergic neuron marker ChAT (scale bar = 100 μm).
ALS Disease Study
Mislocalization of TDP-43 from the nucleus to the cytoplasm is a pathological feature observed in most ALS cases, making it a widely studied readout in ALS disease modeling. Here, iPSCs from a healthy control donor and an ALS patient donor were differentiated into cholinergic neurons using the Quick-Neuron™ Cholinergic – SeV Kit and examined for TDP-43 localization.
TDP-43 localization in healthy control and ALS patient-derived cholinergic neurons. iPSCs from a healthy control donor and an ALS patient donor were differentiated into cholinergic neurons and fixed at Day 10 post-differentiation. Cultures were co-stained for the pan-neuronal marker TUBB3 (green) and TDP-43 (red). Representative images are shown. Punctate TDP-43 signal outside the nucleus was observed in ALS patient-derived neurons.
Neurite Length Analysis
Neurite length is a quantitative, image-based readout commonly used to assess neuronal morphology and outgrowth. Because it can be measured from label-free live-cell images, the same cultures can be monitored over time. Neurite length measurements may be useful for comparing culture conditions, such as healthy versus patient-derived lines or treated versus untreated cultures.
Label-free monitoring of neurite outgrowth over time. (Left) Digital phase contrast (DPC) image of Quick-Neuron™ Cholinergic cells at Day 5, with image-analysis overlays marking somata (blue) and neurites (green); scale bar, 100 µm. (Right) Neurite length measured over 58 days for samples E_02–E_11.
Product Specifications
| Parameters | Specifications |
|---|---|
| Product Name | Quick-Neuron™ Cholinergic - SeV Kit |
| Catalog No. | CH-SeV |
| Product Components | QN-SeV-P, Component N1, Component A, Component P, and Coating Material A |
| Storage Conditions | SeV should be stored at -80°C. All other components can be stored at -20°C or -80°C. |
| Cell Type | Cholinergic Neurons |
| Induction Method | Transcription factors delivered by Sendai virus |
| Differentiation |
At day 7 post-differentiation (CW50065): >80% TUBB3+, >50% ChAT+/TUBB3+ |
| Sterility | No growth observed |
| Mycoplasma | No DNA detected |
| Restricted Use | For research use only. Not for use in diagnostic or therapeutic procedures. |
