Quick Muscle™ Skeletal – SeV Kit
Quick-Muscle™ Skeletal – SeV Kits enable the differentiation of human pluripotent stem cells into skeletal muscle cells using Sendai virus technology through an optimized and reproducible workflow.
Note: This kit is no longer available for individual sale. We now offer bulk orders and custom differentiation services to support your research and development needs.
Advantages of iPSC-Derived Skeletal Muscle
Skeletal Muscle Differentiation Kit Protocol
Our easy-to-follow, optimized skeletal muscle differentiation protocol supports rapid and reproducible differentiation of human induced pluripotent stem cells (iPSCs) into functional skeletal muscle. This step-by-step workflow outlines the key culture stages and timelines for generating skeletal muscle cells from human iPSCs, giving researchers a scalable, reproducible platform for muscle disease modeling, compound screening, and more.
Skeletal Muscle Characterization
Rigorous post-differentiation characterization of our iPSC-derived skeletal muscle cells ensures identity, purity, and functional relevance. Using skeletal muscle–specific markers and phenotypic profiling, we confirm cell lineage and batch-to-batch consistency.
Skeletal Muscle Marker Expression
Expression of skeletal muscle–specific markers is evaluated post-differentiation to verify identity, purity, and maturation status. Using protein-level analyses, we confirm robust expression of myosin heavy chain (MHC), a canonical marker of the contractile apparatus in differentiated skeletal myocytes, to ensure batch-to-batch consistency and experimental reliability.
iPSC-derived skeletal muscle cells express key markers and display typical morphology. Immunofluorescent staining of Quick-Muscle™ Skeletal cells shows typical skeletal muscle cell morphology and MHC expression. Immunofluorescent staining of Quick-Muscle™ Skeletal – mRNA cell cultures on day 6 post-differentiation show expression of myosin heavy chain (scale bar = 100 μm).
Product Specifications
| Parameters | Specifications |
|---|---|
| Product Name | Quick-Muscle™ Skeletal - SeV Kit |
| Catalog No. | SM-SeV |
| Product Components | QMS-SeV, Mesendoderm RNA, Component P, and Coating Material A |
| Storage Conditions | SeV should be stored at -80°C. All other components can be stored at -20°C or -80°C. |
| Cell Type | Skeletal Muscle |
| Induction Method | Transcription factors delivered by Sendai virus |
| Differentiation |
At day 7 post-differentiation (CW50065) >70% of cells express MHC positive cells |
| Sterility | No growth observed |
| Mycoplasma | No DNA detected |
| Restricted Use | For research use only. Not for use in diagnostic or therapeutic procedures. |
