Why do differentiation kits require hPSCs to be maintained under StemFit or StemFlex conditions?
Why doesn’t Solution D1 dissociate my hPSC culture without scraping?
Why are there many aggregates after harvesting hPSCs?
I was able to harvest more hPSCs than I needed for the kit. Can I replate or freeze the leftover hPSCs to maintain their culture?
Can I use Geltrex or Matrigel as a coating substrate for hPSC cultures?
Can I use an orbital shaker for SeV infection while incubating cell suspension for 10 minutes?
Why does my culture have colonies that appear balled up and round on day 1?
I plated cells as instructed, so why does my culture look less confluent than the one shown in the user guide on day 1?
What should I do if I find on day 1 that my culture was seeded only in the center but not in the periphery of the well?
What should I do if I find on day 1 that cells were accumulated in the center of the well?
Why do I see many floating cells and/or cellular debris on day 1?
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