Quick-Neuron™ Excitatory – SeV Kit

Explore our detailed differentiation protocols, a step-by-step guide designed to simplify and optimize your laboratory procedures using our iPSC-derived cells and differentiation kits. These protocols leverage the latest advancements in iPSC technology to ensure efficient and reproducible results.

Excitatory Neuron morphology is confirmed via phase contrast imagery: Representative images of Quick-Neuron™ Excitatory – SeV Kit cell cultures on days 1-10 post-differentiation (scale bar = 100 μm).

Excitatory Neuron Marker Expression

iPSC-derived excitatory neurons express neuronal markers and display typical neurite growth. Immunofluorescent staining of Quick-Neuron™ Excitatory – Human iPSC-derived Neurons on Day 7 post-thaw that shows expression of the pan-neuronal marker TUBB3 and the glutamatergic neuron marker vGLUT1 (scale bar = 100 μm).

Whole-Cell Patch Clamp 

Electrophysiological properties of iPSC-derived excitatory neurons. Whole-cell patch clamp of Quick-Neuron™ Excitatory – Human iPSC-derived Neurons 5-6 weeks post-thaw. (A) A brightfield image of the neurons measured. (B) Spontaneous action potentials were recorded. (C) Spontaneous excitatory postsynaptic current of neurons was detected by voltage clamp measurement at -70mV, indicating the formation of mature synapses. Data courtesy of E-PHY SCIENCE SAS.

Multi-Electrode Array (MEA)

Excitatory Neurons seeded onto MEA plates remain functional after transport. An MEA plate seeded with Quick-Neuron™ Excitatory Neurons and primary human astrocytes was transported from Tokyo, Japan to Maryland, USA. The baseline network burst firing of one well was measured on day 49 post-thaw before transport (A) and on day 50 after 33-hr transport (B). (C) Network burst firing on day 51 exhibited responses to varying concentrations of 4-AP after transport. Data courtesy of Ricoh.

RNA-Sequencing

Gene Expression of Quick-Neuron™ Excitatory Neurons. A heat map of selected gene expression data from RNA-seq performed on undifferentiated iPSCs, Quick-Neuron™ Excitatory Neurons (EX only) cultured for 10 days and 38 days, Quick-Neuron™ Excitatory Neurons cocultured with primary astrocytes (EX+AST) for 18 and 52 days, and primary fetal and adult brain samples is shown. Values represent log10(TPM + 1). Data courtesy of Ricoh.

Principal Component Analysis (PCA)

Quick-Neuron™ Excitatory Neurons display gene expression profiles similar to those of human brain: RNA-seq was performed on undifferentiated iPSCs, Quick-Neuron™Excitatory Neurons (EX only) cultured for 10 days and 38 days, Excitatory neurons cocultured with primary astrocytes (EX+AST) for 18 and 52 days, and primary fetal and adult brain samples. Principal component analysis indicates that Quick-Neuron™ Excitatory Neurons display gene expression similar to that of the human brain, particularly when grown with astrocytes. As cells remain in culture over time they more closely resemble adult human brain cells. Data courtesy of Ricoh.