Quick-Muscle™ Skeletal – SeV Kit
Quick-Muscle™ Skeletal – SeV Kits enable the differentiation of human pluripotent stem cells into skeletal muscle cells using Sendai virus technology through an optimized and reproducible workflow.
Note: This kit is no longer available for individual sale. We now offer bulk orders and custom differentiation services to support your research and development needs.
Advantages of iPSC-Derived Skeletal Muscle
Rapid Differentiation
~1 week
Optimized & Reproducible
ISO-9001 certified
Highly Pure Population
MHC+
No Genetic Footprint
0 modifications
Skeletal Muscle Differentiation Kit Protocol
Explore our detailed differentiation protocols, a step-by-step guide designed to simplify and optimize your laboratory procedures using our iPSC-derived cells and differentiation kits. These protocols leverage the latest advancements in iPSC technology to ensure efficient and reproducible results.
Skeletal muscle morphology is confirmed via phase contrast imagery: Representative phase contrast images of Quick-Muscle™ Skeletal – mRNA Kit cell cultures on days 1-6 post-differentiation (scale bar = 100 μm)
Skeletal Muscle Characterization
Characterization of iPSC-derived skeletal muscle is crucial to ensure their utility in research. Employing skeletal muscle markers, we can confirm the identity and purity of these neurons.
Skeletal Muscle Marker Expression
Understanding the role of skeletal muscle markers is crucial in neuroscience research. Our comprehensive guide delves into the identification and significance of these markers in iPSC-derived muscle cells, providing essential information for researchers.
iPSC-derived skeletal muscle cells express key markers and display typical morphology. Immunofluorescent staining of Quick-Muscle™ Skeletal cells shows typical skeletal muscle cell morphology and MHC expression. Immunofluorescent staining of Quick-Muscle™ Skeletal – mRNA cell cultures on day 6 post-differentiation show expression of myosin heavy chain (scale bar = 100 μm).
Product Specifications
| Parameters | Specifications |
|---|---|
| Product Name | Quick-Muscle™ Skeletal - SeV Kit |
| Catalog No. | SM-SeV |
| Product Components | QMS-SeV, Mesendoderm RNA, Component P, and Coating Material A |
| Storage Conditions | SeV should be stored at -80°C. All other components can be stored at -20°C or -80°C. |
| Cell Type | Skeletal Muscle |
| Induction Method | Transcription factors delivered by Sendai virus |
| Differentiation |
At day 7 post-differentiation (CW50065) >70% of cells express MHC positive cells |
| Sterility | No growth observed |
| Mycoplasma | No DNA detected |
| Restricted Use | For research use only. Not for use in diagnostic or therapeutic procedures. |
Skeletal Muscle Resources
Quick-Muscle™ Skeletal – SeV Kit
Characterization of iPSC-derived Skeletal Muscle Generated with Rapid Differentiation Method (Japan Muscle Society 2021)
Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.
Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells.
Therapeutic Genome Editing for Myotonic Dystrophy Type 1 Using CRISPR/Cas9.
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FAQs
Do I need a license agreement for any of Ricoh Biosciences’ products?
No. You don’t need any licence or material transfer agreement (MTA) to use our differentiation kits or iPSC-derived cells. However, please be advised that these products are for research use only.
What kind of transcription factors are used for differentiation induction?
It is a proprietary formulated RNA and cannot be disclosed.
Does Quick-Tissue™ technology leave a genetic footprint?
Sendai virus (SeV) is an RNA virus, so it does not integrate into the genomic DNA. In principle, a foreign gene introduced intracellularly in the form of RNA is quickly translated and expressed because, unlike DNA, RNA does not need to enter the nucleus for forced expression, thereby providing no chance of mutagenesis. This is discussed in the following review paper: Yamamoto, et al., (2009) “Current prospects for mRNA gene delivery.” Eur. J. Pharm Biopharm 71, 484-489.
Will SeV remain active after differentiation?
No. The SeV used in our kits is a temperature-sensitive mutant that is active at 33℃ but becomes inactive at 37℃, which is the temperature instructed in the user guides post-differentiation.
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