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Inside this application protocol
Review a complete workflow for transfecting adherent somatic cells with mRNA, including all required reagents, protocol steps, and representative expression data.
- Step-by-step transfection workflow compatible with adherent somatic cells, including human dermal fibroblasts and HEK293 cells, in a 24-well plate format.
- Detailed reagent volumes and critical timing guidance for assembling the mRNA-Lipofectamine MessengerMAX complex with B18R-supplemented medium.
- Appendix fluorescence imaging data from HDFn and HEK293T cells transfected with a range of modified EGFP mRNAs, with expression quantified at multiple timepoints post-transfection.
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