Poster
2D and 3D iPSC-Derived Platforms for Neurotoxicity Screening Demonstrate Compound Effects on Calcium Activity, Synapses, Viability, and Cell Proliferation
Validated 2D and 3D iPSC-derived neural progenitor, tri-culture, and spheroid platforms quantify calcium activity, synapse changes, viability, and proliferation for drug and toxicant neurotoxicity screening.
Download Your Poster
"*" indicates required fields
We respect your inbox. You can unsubscribe at any time. View our privacy policy.
Inside This Poster
Examine validated iPSC-derived neural progenitor, tri-culture, and 3D spheroid platforms used to screen antiretroviral, chemotherapy, and environmental toxicant compounds for neurotoxic effects on calcium activity and viability.
- Exposure to elvitegravir and dolutegravir alone or combined decreased neural progenitor cell counts, while tenofovir disoproxil fumarate increased the percent of dividing cells, assessed across up to 25 wells per condition.
- Isogenic tri-cultures combining hiPSC-derived neurons, astrocytes, and microglia at a 70:20:10 ratio showed reduced neuron spike integral, frequency, and amplitude after 24 to 48 hour exposure to dolutegravir and lead chloride.
- Ten week old 3D neurospheres containing microglia, plated on flat bottomed 384 well plates and imaged at 4 frames per second, showed distinct single cell calcium transients across the spheroid.
Related Resources
Explore related cell models, services, and protocols connected to this application.