Quick-Neuron™ Precursor – Human iPSC-Derived Neural Precursor Cells (Healthy Donor)
Quick-Neuron™ human iPSC-derived neural precursor cells are expandable, multipotent neural progenitors that provide a versatile in vitro model of early human neural development. These cryopreserved, ready-to-use iPSC-derived precursor cells enable reproducible differentiation into multiple neural lineages and support applications in neurodevelopmental studies, cell fate specification, disease modeling, and compound screening.
$1,100.00
Advantages of iPSC-Derived Neural Precursor Cells
Rapid Differentiation
~1 week
Functionally Validated
QC confirmed
Highly Pure Population
Nestin+, Vimentin+
No Genetic Footprint
0 modifications
Neural Precursor Cells Protocol
Explore our detailed differentiation protocols, a step-by-step guide designed to simplify and optimize your laboratory procedures using our iPSC-derived cells and differentiation kits. These protocols leverage the latest advancements in iPSC technology to ensure efficient and reproducible results.
Representative phase contrast images of Quick-Neuron™ Precursor – Human iPSC-derived Neural Precursor Cells on days 1-7 post-thaw (scale bar = 100 μm). Cells are ready for passaging at Day 7.
Neural Precursor Cells Characterization
Characterization of iPSC-derived neural precursor cells is crucial to ensure their utility in research. Employing neural precursor markers, we can confirm the identity and purity of these cells.
Neural Precursor Cell Marker Expression
Understanding the role of neural precursor markers is crucial in neuroscience research. Our comprehensive guide delves into the identification and significance of these markers in iPSC-derived neural precursor cells, providing essential information for researchers.
Immunofluorescent staining of Quick-Neuron™ Precursor – Human iPSC-derived NPCs shows expression of nestin and vimentin on day 13 post-thaw (scale bar = 100 μm). Staining conditions: Anti-Nestin Antibody, clone 10C2 (Millipore Sigma, Catalog Number: MAB5326, 1:500 dilution) was used in combination with a secondary antibody (Invitrogen, Catalog Number: A11032 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594, 1:500 dilution). Recombinant Anti-Vimentin antibody [EPR3776] – Cytoskeleton Marker (abcam, Catalog Number: ab92547, 1:500 dilution) was used in combination with a secondary antibody (Invitrogen, Catalog Number: A32731 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488, 1:500 dilution). Nuclei were counterstained with Hoechst 33342.
Real-time Quantitative PCR Analysis
Real-time quantitative PCR analysis of expression levels of neuronal precursor-associated genes NES, VIM, HES5, SOX1 and SOX2, as well as the stem cell pluripotency marker POU Class 5 Homeobox 1 (POU5F1/OCT4), were examined. Graph shows gene expression in Quick-Neuron™ Precursor Culture on day 6. The relative gene expression is normalized to phosphoglycerate kinase 1 (PGK1), and then calculated as a fold induction relative to undifferentiated hiPSCs as a control. Error bars show standard deviation.
Product Specifications
| Parameters | Specifications |
|---|---|
| Product Name | Quick-Neuron™ Precursor – Human iPSC-derived Neural Precursor Cells |
| Catalog No. | NP-mRNA-HC-CW50065 |
| Product Components | Cryopreserved cells |
| Starting Material | iPSCs derived from peripheral blood mononuclear cells (CIRM line CW50065) |
| Storage Conditions | Frozen cells should be stored in liquid nitrogen (vapor phase). The rest of the components should be stored at -20°C. |
| Cell Type | Neural Precursor Cells |
| Culture Type | Feeder Cell-Free |
| Disease | Healthy Donor |
| Donor Sex | Female |
| Donor Age at Sampling | 74 |
| Donor Race Ethnicity | Caucasian, not Latino |
| Patient History | See Resources for more information. |
| Reprogramming Method | Episomal vector |
| Induction Method | Transcription factors delivered by synthetic mRNA |
| Growth Properties | Adherent |
| Number of viable cells | > 1.0 million viable cells per vial upon thawing |
| Cell viability and remaining live cells |
>50% at day 1, >211 live cells per mm2 >50% at day 7, >211 live cells per mm2 |
| Differentiation |
At Day 7 Post-Differentiation >80% Nestin+, >80% Vimentin+ |
| Sterility | No growth observed |
| Mycoplasma | No mycoplasmal enzymes detected |
| Morphological Observation | Cells are adherent and exhibit a uniform, bipolar or multipolar morphology, indicative of a proliferative neural precursor phenotype. |
| Restricted Use | For research use only. Not for use in diagnostic or therapeutic procedures. |
Neural Precursor Cell Resources
2D and 3D iPSC-Derived Platforms for Neurotoxicity Screening Demonstrate Compound Effects on Calcium Activity, Synapses, Viability, and Cell Proliferation (Society for Neuroscience 2023, Vala Sciences)
Anti-retroviral therapy is toxic to iPSC-derived NPC’s in vitro and alters neuronal calcium transients in differentiated NPCs (Society for Neuroscience 2023, Vala Sciences)
No results found.
Related Products
FAQs
Does Quick-Tissue™ technology leave a genetic footprint?
Sendai virus (SeV) is an RNA virus, so it does not integrate into the genomic DNA. In principle, a foreign gene introduced intracellularly in the form of RNA is quickly translated and expressed because, unlike DNA, RNA does not need to enter the nucleus for forced expression, thereby providing no chance of mutagenesis. This is discussed in the following review paper: Yamamoto, et al., (2009) “Current prospects for mRNA gene delivery.” Eur. J. Pharm Biopharm 71, 484-489.
Will SeV remain active after differentiation?
No. The SeV used in our kits is a temperature-sensitive mutant that is active at 33℃ but becomes inactive at 37℃, which is the temperature instructed in the user guides post-differentiation.
Is Sendai virus (SeV) dangerous?
SeV is not pathogenic to humans (i.e., humans are not the natural host of the virus) and the infection does not persist in immunocompetent animals. Furthermore, SeV used in our kits does not produce infectious viral particles upon transduction to host hPSCs and is a temperature-sensitive mutant, such that it is active at 33℃ but becomes inactive at 37℃. However, because SeV can be transmitted by aerosol and contact with respiratory secretions and is highly contagious, appropriate care must be taken to prevent potential mucosal exposure to the virus. Our SeV-based kits must be used under Biosafety Level 2 (BL-2) containment with a biological safety cabinet or a laminar flow hood and with appropriate personal protective equipment. In the event that the virus comes into contact with skin or eyes, decontaminate the affected area by flushing with plenty of water and follow the safety manual prepared by your laboratory and approved by your Institutional Biosafety Committee.
Do I need a license agreement for any of Ricoh Biosciences’ products?
No. You don’t need any licence or material transfer agreement (MTA) to use our differentiation kits or iPSC-derived cells. However, please be advised that these products are for research use only.
No results found.
Contact Us
Have a question about our products, services, or custom projects? Our team is here to help—reach out and we’ll get back to you as soon as possible.
Subscribe
Sign up to our eNewsletter to stay up to date with the latest product launches, promotions, and receive expert tips.
By signing up you are agreeing to our Privacy Policy