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Inside this application protocol
Review a complete workflow for plating, maturing, and assaying 3D iPSC-derived neuronal spheroids co-cultured with human primary astrocytes for pharmacological calcium flux screening on the FDSS/μCELL platform.
- Spontaneous Ca2+ oscillations are detectable approximately 3 weeks after plating, with drug responses measurable within 6 weeks when iPSC-derived neurons are co-cultured with human primary astrocytes.
- The protocol supports 120 spheroids per half 384-well plate using 8,000 neurons and 8,000 astrocytes per spheroid at a 1:1 ratio, with compatibility for automated liquid handling platforms including ASSIST PLUS and Agilent Bravo.
- Dose-dependent Ca2+ responses were recorded across multiple compounds including 4-Aminopyridine, Carbamazepine, GABA, bicuculline, glutamate, and kainic acid, with area-under-curve analysis performed 40 minutes post-administration.
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